Comparative study of antioxidant effects of different alcoholic and oil extracts of dry dates and leaves (Phoenix dactylifera L) palm against CCL4 induced oxidative stress in rats
Pages : 613-617Download PDF
A study was performed to investigate the protective effect of different extracts of alcoholic and oil of leave and dry date of ( Phoenix dactylifera) against oxidative stress induced by CCL4 at a dose of 100 mg/kg given orally daily to five treated groups (CCl4 control (+ve) , alcoholic extract of leave (150 mg/kg) , alcoholic extract of dry date (100 mg/kg) , oil extract of leave and date (250 mg/kg), also two control dosed with vehicles (distal water and corn oil) were used . At the end of two months experiment blood were collected and oxidant and antioxidant parameter were studied in serum of all animal groups including Malon dialdehyte (MDA) and reduce glutathione (GSH). Furthermore ,the possibility of genomic polymorphism in glutathione-S- transference enzyme (GSTs) isomers (GSTT1 and GSTM1) were studied in all animal groups blood by using conventional PCR technique to understand their involvement in oxidative toxicity induced by CCL4 alone and with different palm date and leave extracts.` The biochemical results recorded a significant increase in serum MDA with significant decrease in reduce glutathione (GSH) only in CCL4 treated group compared to other extract treated one or control groups indicating lipid perioxidation effect induced by CCL4 and improvement effect of such effect by all used date palm extracts. Also polymorphism of GSTs isomers were recorded only in CCL4 group genomic result manifested by absence of GSTM1 isomer that indicate reduction in GSTs enzyme function and its involvement as one of the main antioxidant enzyme while the appearance of both GSTT1 and GSTM1 in genomic results of different extract treated groups were indicative of antagonistic ability of different date palm extract to oppose oxidative effect that cause improvement in oxidant / antioxidant balance nearly as that of control groups.
Keywords: Phoenix dcactylifera, leave, dry date, oxidative stress, antioxidant, polymorphism