Article Published In Vol.3 (Sept-Oct-2015)

Evaluation of Mesenchymal Stem Cells and Vitamin E in Treatment of Infertile Male Albino Rats

Author : Hussein YM1, Hussein RM, Amin AI Mohamed AS ,Hussein HS

The present study describes the effect of mesenchymal stem cells and vitamin E on infertility of adult male rats. Induction of infertility by oral administration of cadmium chloride (cdcl₂) for 30 days. Induction of infertility caused a reduction in body weight as well as in genital sex organs weight. The epididymal sperm counts and their motility were significantly decreased following cdcl₂ administration, while significantly increased in fructose in semen. A reduction in serum levels of luteinizing hormone (LH) and testosterone, and an elevation in serum follicle stimulating hormone (FSH) and prolactin (PRL) were also noticed in infertile rats. Significant increases in malondialdehyde (MDA), nitric oxide (NO) and catalase (CAT) levels were observed in testicular homogenate of infertile rats. Decreased in reduced glutathione (GSH) content, Superoxide dismutase (SOD) and total antioxidant were also found in induced infertility rats. Results showed that stem cells significantly increased of SOD in testes, LH and total antioxidant in serum, fructose in semen and fix all disruption caused by induction of infertility. Results demonstrated the beneficial influences of stem cells and vitamin E in reducing the harmful effects of cdcl₂ while vitamin E was less effective than stem cells. DNA fragmentation of testes from experimental animals was examined. Liver functions, kidney function, were estimated.

Keywords: Infertility; Cdcl₂; mesenchymal stem cells; vitamin E; antioxidant; SOD ; NO CAT ; GSH ; MDA ; LH ; FSH ; PRL ; testis and DNA fragmentation.