Genotyping of Human Papilloma Virus (HPV) Isolated from Breast Cancer Patients in Radiation and Isotopes Center Khartoum (RICK)–Sudan
Pages : 590-597Download PDF
Background: Breast cancer is the most common among female comprises about (18%) of all female cancers, with (1.7) million new cases in the world each year. Recently some studies reported that approximately (18%) of cancer cases can be linked to infectious agents including viruses particularly Human Papilloma Virus (HPV).
Objectives: The objectives of this study were to investigate of which genotype of HPV is implicated as well as the risk factors associated with HPV in breast cancer by using PCR as diagnostic tools for HPV in breast tissues.
Materials and methods: One hundred forty three females diagnosed with histopathological examinations to have breast cancer (n=100 patients) or breast inflammatory conditions (n=43patients) were employed in this study. Tissue sample of (10) μm was taken from the pathological tissues of each patient. The samples were subjected to a PCR protocol to determine whether the patient is infected with HPV or not. HPV infected samples were further subjected to anther PCR protocol to identify the genotypes of HPV. The risk factors associated with breast cancer were taken from patient’s records. The data were subjected to analytical and descriptive statistical analysis.
Results & Discussion: Out of the cases screened for breast pathology, 41(29%) patients were found to have, invasive ductal carcinoma, 21(15%) invasive lobular carcinoma, 12(8%) invasive micropapillary carcinoma, 26(18%) medullary carcinoma and 43(30%) have inflammatory breast conditions.
Genotyping of HPV infected breast cancer specimen was performed. Out of the (143) specimens (breast cancer and breast inflammatory conditions) the following genotypes were determined and mentioned in a descending order. 22(33%) of the HPVs were of the genotype 16, 21(31%) genotype 18=14(21%) genotype 33=14(21%) and 10(15%) genotype (31).
Conclusion: In conclusion, the findings of this study provide strong association between HPV genotypes and BC in Sudan.
Keywords: HPV, Breast cancer, RICK, Sudan, PCR, Cell cycle.