Immunoinformatics Approach for Designing Epitope-Based Peptides Vaccine of L1 Major Capsid Protein against HPV Type 16Download PDF
Human papilloma viruses (HPV) are small DNA, non-enveloped, double-stranded and closed circular viruses. There are more than 150 HPV identified types. Genital HPV types are categorized according to their epidemiologic association with cervical cancer to high and low risk types. The high risk type HPV 16 is the most common in the world. we aimed to design a universal peptide based vaccine against HPV type 16 virus using Immunoinformatics Approach through prediction of highly conserved T and B-cell epitopes from the most abundant and highly immunogenic protein (L1 major capsid protein) derived from HPV type 16 strains all over the world. All sequences of the L1 major capsid protein were retrieved from NCBI database. Potentially continuous B and T cell epitopes were predicted using tools from immune epitope data base analysis resource (IEDB-AR). The Allergenicity of predicted epitopes was analyzed by AllerTOP Tool and the coverage was determined throughout the worlds. The B cell epitope 243KSEV246 and T cell epitopes 363TRSTNMSLC371, 470YTFWEVNLK478, 260YIKMVSEPY268 and 236FTTLQANKS244 were suggested to become universal peptide based vaccine against HPV type 16. We hope to confirm our findings by adding complementary steps of both in vitro and in vivo studies to support this new universal predicted vaccine.
Keywords: Human papilloma viruses (HPV), Epitope, vaacine & In Silico.