Effect of Gibberellic acid and Trichoderma SPP efficiency on seeds germination, speed Percentage, the activity of Peroxidase and Amylase enzyme of two eggplant (Solanum melongena) Hybrids

Abstract

The experiment was implemented in the laboratory of Department of Biology, Faculty of Education .To detect the ability of Trichoderma as bio-fertilizer and Gibberellic acid (GA3) at concentration of (5 and10) mg.l-1 to improve seeds germination  of two eggplants hybrids (Solanum melongena) (A) French origin and (B) Syrian origin which were treated with (GA3) at concentration of (5,10) mg.l-1and Trycoderma as  biofertilizer  in addition to distilled water as control treatment for (15min) of period. Seeds germination percentage and speeds rate, plus Peroxidase and Amylase enzyme activity as parameters were recorded. Completely randomized design (C.R.D.) was used and Data analysis performed using GenStat program software. 3 replicates (plates) with 20 seeds in each plate. Means were compared based on Least Significant Differences (L.S.D.)   at P ≤ 0.05 level. The results revealed that (B) hybrid was superior in seeds germination percentage and  Peroxidase activity  which reached (94.4%,50.5 U/ml) as compare with (A) hybrid which was superior to give highest  seeds germination speed 13.1 day .Moreover, treated seeds with 10 mg.l-1 of  Gibberellic acid affected significantly on seeds speed , germination percentage and activity of amylase enzyme which reached (92.5%,8.6 day and 57.4 U/ml) with reduction in Peroxidase activity percentage reached (23.2 U/ml) as compare with control treatment.